Nucleofill is two forms of a product family in the polynucleotide (PN) class: polynucleotides purified from salmon DNA. Soft Plus is prepared for the eye area, Strong Plus for the full face, neck and décolletage. It is commonly known as the “DNA Glow”. It is not a filler; it adds no volume and does not reshape the face. Within this class I work with products that have traceability and a certificate of analysis; this page is an information page for anyone looking into Nucleofill.
The general framework of the polynucleotide class — the difference from PDRN, the level of evidence, where the lines are between classes, the comparison of product families — is on the salmon DNA (polynucleotide) treatment page. Here I cover Nucleofill only.
Two forms
Nucleofill Soft Plus. The form prepared for the thin skin of the eye area; according to the manufacturer it is more fluid. It is also sold under the name “Nucleofill Eyes”. Used under the eyes for thin skin, dark circles and crepey texture.
Around the eyes the decision depends on the cause: if hollowing dominates, mesotherapy is not enough and filler is discussed; if thin skin, dark circles and mild puffiness dominate, HA–amino acid biorevitalisers or polynucleotide products come forward. The product is chosen at the consultation.
The whole of the under-eye picture — the sources of dark circles, blood tests, the boundary with light filler — is on the under-eye polynucleotide treatment page; the decision framework there applies to Soft Plus as well.
Nucleofill Strong Plus. The form prepared for the full face, neck and décolletage; according to the manufacturer it is the more concentrated form of the family. It is placed in the deep dermis using mesotherapy technique, by spread micro-injection. Used for loss of elasticity, dullness, superficial scarring and horizontal lines on the neck and décolletage. Which method leads for which problem on the neck and décolletage is on the neck and décolletage rejuvenation page.
Among products in the same class the difference is decided by indication, not brand; with Nucleofill too, I make the decision at consultation.
How does it work?
The product is placed in the dermis by spread micro-injection or with a cannula. Long-chain polynucleotides are reported to form a water-holding network that provides structural support and to play a role in remodelling the extracellular matrix 2. It has been suggested that the receptor mechanism described for short-chain PDRN also applies to polynucleotides, but this has not yet been demonstrated scientifically 1.
To be open about the evidence: alongside studies reporting improvement in skin texture and elasticity with polynucleotides, the literature includes studies reporting limited or no benefit 1. That is why I give no percentages. The product does not remain in the tissue; it does its repair work and is metabolised.
Who is it suitable for?
- Thin skin, dark circles and crepey texture under the eyes (Soft Plus)
- Dull skin beginning to lose elasticity (Strong Plus)
- Superficial acne scarring and pore visibility (Strong Plus)
- Horizontal lines and thinning on the neck and décolletage (Strong Plus)
- Anyone who does not want volume and wants their face shape left alone
Session plan and what to expect
- 3 sessions, 3 weeks apart. The same protocol for both forms.
- Hydration and a sense of relief after the first session; the tissue effect becomes visible after the second.
- The effect lasts around 9 months. Maintenance is a single session, planned by tissue response rather than by calendar.
The procedure and pain management
The procedure takes 15–20 minutes. Soft Plus is placed around the eyes with a fine needle or cannula; Strong Plus is placed in the deep dermis of the face, neck and décolletage by spread micro-injection.
Topical anaesthetic cream is standard. Because I am an anaesthesiologist, I treat pain management as a separate part of the procedure. The procedure is well tolerated; a mild pressure sensation is normal.
Aftercare
First 24 hours
- No make-up on the treated area
- No hot showers, sauna, hammam or steam
- Avoid heavy exercise
- No alcohol
- Do not sleep face-down (facial treatments)
- Do not touch the injection points; do not squeeze or massage the bumps
First 48 hours
- Avoid direct and prolonged sun exposure
- No facial massage, skincare treatments or energy-based device treatments
Expected findings Small bumps (papules) at the injection points lasting 12–24 hours are expected and show that the product has settled in the skin. Mild swelling, tenderness or bruising lasting a few days may occur. Around the eyes, bruising is slightly more likely than on the rest of the face.
Safety and anatomy
The products are salmon-derived and produced at high purity. Papules, temporary swelling, tenderness and bruising can occur at injection points. The eye area is densely vascular; using a fine needle or cannula lowers the chance of bruising. Polynucleotide has a low tendency to hold water and cause swelling. Polynucleotide is not a substance that is dissolved with an enzyme the way hyaluronic acid fillers are; it does not remain in the tissue and is metabolised. Sterile technique is used, and treatment is postponed if there is an active infection.
Because allergic reactions can occur rarely, treatments are carried out under medical supervision.
Product and regulatory status: Nucleofill Soft Plus and Strong Plus have been assessed under CE and TİTCK frameworks for their relevant indications. Approval belongs to the product and to a specific indication, not to a technique or to a physician.
Who is not suitable
Treatment is not performed, or is postponed, in the following situations:
- Active infection or inflammation in the treatment area
- Autoimmune disease in an active phase
- Pregnancy and breastfeeding
- Known hypersensitivity to the product components
- Uncontrolled chronic illness
- Untreated bleeding disorder or unregulated anticoagulant use
Because the product is salmon-derived, treatment is also not performed in people with a known serious allergy to fish or salmon protein.
Without being a medical contraindication, I also consider expectation and treatment to be mismatched in these situations:
- People expecting a marked and immediate change from a single session
- People with under-eye hollowing — polynucleotide does not fill a hollow
- People with advanced tissue laxity who require surgical assessment — no polynucleotide product replaces a thread lift or surgery
Suitability is determined by consultation in every case.
Pricing
Under the applicable regulations, price information cannot be published on our website. The cost of a treatment depends on the product used, the technique chosen and the extent of the treatment. You can call the clinic for detailed information.
Frequently asked questions
What is the difference between Nucleofill Soft Plus and Strong Plus?
Soft Plus is the form prepared for the thin skin of the eye area; Strong Plus is the form prepared for the full face, neck and décolletage and is placed in the deep dermis. The decision depends on the area; the final choice is made at consultation.
Why is Nucleofill called the DNA Glow?
Because it is derived from salmon DNA and used with hydration and radiance as the goal, it has picked up this name. The name has no medical meaning; the product class is polynucleotide.
Are Nucleofill Eyes and Soft Plus the same?
Yes. The same product is sold under the names “Nucleofill Eyes” and “Nucleofill Soft Plus”; on this page I use the name Soft Plus.
Which areas is Nucleofill used on?
Soft Plus is used around the eyes; Strong Plus on the full face, neck and décolletage. Areas that need shaping and volume are not this product’s job; that need is assessed for filler.
Planning your visit
My clinic is in Nişantaşı, Şişli, on the European side of Istanbul, approximately 45 minutes from Istanbul Airport.
For a Nucleofill treatment I suggest planning a stay of two to three days: arrival, consultation and treatment, and departure. Because this treatment is planned as three sessions three weeks apart, it requires more than one visit. The first session takes place in Istanbul, and we plan the schedule of the following sessions together according to your travel arrangements, with remote follow-up in between. Flying is generally appropriate 24 hours after an injectable treatment.
Consultations are conducted in English. Follow-up after you return home is arranged remotely, and I remain reachable for any questions during the period in which the result develops.
Dr. Ömer Fatih Şahin — Specialist in Anaesthesiology and Reanimation Diploma Registration No: 113606
Published: 2026-09-21 · Last updated: 2026-09-21 This content has been medically reviewed by Dr. Ömer Fatih Şahin.
References
- Lee KWA, Chan KWL, Lee A, et al. Polynucleotides in Aesthetic Medicine: A Review of Current Practices and Perceived Effectiveness. Int J Mol Sci. 2024;25(15):8224. https://pubmed.ncbi.nlm.nih.gov/39125793/
- Kim ST. Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives. Pharmaceutics. 2025;17(8):1024. https://pubmed.ncbi.nlm.nih.gov/40871045/